| CreateAssayObject {SeuratObject} | R Documentation |
Create an Assay object from a feature (e.g. gene) expression matrix. The expected format of the input matrix is features x cells.
CreateAssayObject(counts, data, min.cells = 0, min.features = 0, ...)
counts |
Unnormalized data such as raw counts or TPMs |
data |
Prenormalized data; if provided, do not pass |
min.cells |
Include features detected in at least this many cells. Will subset the counts matrix as well. To reintroduce excluded features, create a new object with a lower cutoff. |
min.features |
Include cells where at least this many features are detected. |
... |
Arguments passed to |
Non-unique cell or feature names are not allowed. Please make unique before calling this function.
A Assay object
## Not run:
pbmc_raw <- read.table(
file = system.file('extdata', 'pbmc_raw.txt', package = 'Seurat'),
as.is = TRUE
)
pbmc_rna <- CreateAssayObject(counts = pbmc_raw)
pbmc_rna
## End(Not run)