| findrRNA {microseq} | R Documentation |
Finding rRNA genes in genomic DNA using the barrnap software.
findrRNA(genome.file, bacteria = TRUE, cpu = 1)
genome.file |
A FASTA file with the genome sequence(s). |
bacteria |
Logical, the genome is either a bacteria (default) or an archea. |
cpu |
Number of CPUs to use, default is 1. |
The external software barrnap is used to scan through a prokaryotic genome to detect the rRNA genes (5S, 16S, 23S). This free software can be installed from https://github.com/tseemann/barrnap.
A GFF-table (see readGFF for details) with one row for each detected
rRNA sequence.
The barrnap software must be installed on the system for this function to work, i.e. the command system("barrnap --help") must be recognized as a valid command if you run it in the Console window.
Lars Snipen and Kristian Hovde Liland.
## Not run:
# This example requires the external barrnap software
# Using a genome file in this package.
genome.file <- file.path(path.package("microseq"),"extdata","small.fna")
# Searching for rRNA sequences, and inspecting
gff.tbl <- findrRNA(genome.file)
print(gff.table)
# Retrieving the sequences
genome <- readFasta(genome.file)
rRNA <- gff2fasta(gff.tbl, genome)
## End(Not run)